Charlie – who presented an award for the Outstanding Actor in a Comedy Series, which he called his "old category" - announced: "I want to take a moment to get something off my chest ad say a few words to everybody here from 'Two and a Half Men'.
"From the bottom of my heart I wish you nothing but the best for this upcoming season. We spent eight wonderful years together … and I know you will continue to make great television."
Earlier in the night, the 46-year-old actor's former co-star Jon Cryer revealed he has "converted" to be a fan of Charlie's replacement because he's "totally different".
Speaking to Ryan Seacrest at the ceremony, he said: "I'm already converted to the cult of Ashton. I like him. He's younger, taller – it's a totally different thing."
While presenting the Outstanding Supporting Actress and Best Writing in a Drama Series with Jon, Ashton joked: "I am not Charlie Sheen. And Jon, I want to tell you something. I do not think you are a troll."
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