The 'Anger Management' star - who has five children - reportedly made the generous donation to help a 10-year-old girl called Jasmine, who is battling an aggressive form of muscle tissue cancer known as Childhood Rhabdomyosarcoma, after meeting her police officer father on Hermosa Beach in Los Angeles recently and hearing of her illness.
A source told gossip website TMZ.com that the actor told the police officer: "no parent should have to watch their kid go through that" and sent a cheque to the police department just one day later, who have started a fund to raise money for the little girl who requires over a year of expensive medical treatments, including chemotherapy and radiation.
The generous actor's stunt-double Eddie Braun is also said to have sent a cheque for $25,000 to the fund after hearing about Jasmine through his good pal.
Charlie has donated a significant amount of money to various charities recently.
The former 'Two And A Half Men' star - who reportedly sent his co-star Lindsay Lohan a cheque for $100,000 to cover almost half of her hefty tax bill after they bonded while filming 'Scary Movie 5' together - is said to have given the rest of his fee to charity.
A source close to the 47-year-old star previously revealed that he was paid a total of $250,000 for one day's work on the movie in September.
And the insider revealed that the 26-year-old actress wasn't the only person to benefit because he donated the remaining $150,000 to three different charities.